Dot-enzyme-linked immunosorbent assay (Dot-ELISA) for detection of pneumococcal polysaccharide antigens in pleural fluid effusion samples.: Comparison with bacterial culture, counterimmunoelectrophoresis and latex agglutination
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چکیده
منابع مشابه
Dot enzyme-linked immunosorbent assay (dot-ELISA) for schistosomiasis diagnosis using dacron as solid-phase.
Dacron and nitrocellulose were evaluated as matrices for the dot enzyme linked immunosorbent assay (dot-ELISA) for schistosomiasis and compared to indirect immunofluorescence (IMF). Titration of sera from 18 schistosomiasis patients against soluble worm antigen preparation (SWAP) was carried out and sera from healthy individuals from non-endemic areas were used as controls. The IMF was less sen...
متن کاملComparison of Dot-ELISA with microbial culture for detection of Brucella spp. in clinical specimens
Definitive diagnosis of brucellosis is made by isolation of the causative agents, which is a timeconsumingprocedure. To evaluate the efficacy of Dot-ELISA for detecting brucellae in clinical samples, 94different specimens taken from animal origin were cultured on brucella selective culture media and colonieswere identified biochemically. The specimens were also examined after centrifugation by ...
متن کاملComparison of a latex agglutination assay and an enzyme-linked immunosorbent assay for detecting cholera toxin.
To determine the pandemic potential of Vibrio cholerae, one must demonstrate both the presence of O1 antigen and the production of enterotoxin (CT). Tissue culture or enzyme-linked immunosorbent assays (ELISAs) for CT have been limited to research and reference laboratories. A kit for detecting CT by reversed passive latex agglutination is now commercially available and was used to test 168 str...
متن کاملEvaluation of dot enzyme-linked immunosorbent assay for mucocutaneous leishmaniasis and comparison with microplate enzyme immunoassay.
A dot enzyme-linked immunosorbent assay (dot ELISA) was evaluated and compared with a standard microplate ELISA (immunoglobulin G [IgG] ELISA) for the serological diagnosis of mucocutaneous leishmaniasis. The two assays were used to test 113 serum specimens from the following groups: normal individuals and patients with deep mycoses, toxoplasmosis, mucocutaneous leishmaniasis, visceral leishman...
متن کاملDiagnostic tests for amoebic liver abscess: comparison of enzyme-linked immunosorbent assay (ELISA) and counterimmunoelectrophoresis (CIE).
The liver abscess is the most frequent extraintestinal complication of intestinal amoebiasis: its diagnosis is suggested by the clinical picture but it must be confirmed by paraclinic tests. Themost stringent diagnosis requires identification of E. histolytica. But this is possible only in a few cases. Serological tests greatly improve the diagnosis of this severe complication of amoebiasis. We...
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ژورنال
عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo
سال: 1994
ISSN: 0036-4665
DOI: 10.1590/s0036-46651994000600010